Mouse IgG2a ELISA Kit
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Product name IgG2a, ELISA kit Full product name IgG2a Isotyping (mouse) ELISA Product gene name IgG2a Elisa kit For research use only For research use only. It should not be used in diagnostic procedures. Species reactivity: Mouse Preparation and Storage: 2 – 8 degrees C Product note Information selected from the online data sheet is…
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Mouse IgG1 ELISA Kit
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Properties Species reactivity: mouse Technique(s) ELISA: adequate Test range cv between trials: <10% intra-assay cv: <12% Sent in: wet ice Storage temperature: −20°C Product description Abcam’s in vitro Mouse IgG1 ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the precise quantitative measurement of Mouse IgG1 in culture supernatants and serum. IgG1-specific antibody was pre-coated onto…
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Insulin ELISA Kit
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Overview Product name Human Insulin ELISA Kit Detection method: colorimetric Example type Cell culture supernatant, Serum, Plasma Essay type: sandwich (quantitative) Sensitivity: < 4 µIU/mL Distance: 4.69 µlU/ml – 300 µlU/ml Recovery: 89% Test duration: Standard multi-step assay Species reactivity Reacts with: Human Intended to work with: Mouse, Rat, Cow, Pig Product description Abcam’s Human…
Read MoreDMPK on Recombinant Adeno-Associated Virus-Based Gene Therapy: Past Learning, Current Support, and Future Contribution
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The recent approvals of Luxturna and Zolgensma prove that recombinant adeno-associated virus (rAAV)-based gene therapy is a transformative modality that enables curative treatment for genetic disorders. Over the last decade, Takeda has accumulated significant experience with rAAV-based gene therapies, especially in the early stage of development. In this review, based on the learnings from Takeda and…
Read MoreDevelopment of a Novel Double Antibody Sandwich ELISA for Quantitative Detection of Porcine Deltacoronavirus Antigen
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Porcine deltacoronavirus (PDCoV) can cause diarrhea and dehydration in newborn piglets. Here, we developed a double antibody sandwich quantitative enzyme-linked immunosorbent assay (DAS-ELISA) for detection of PDCoV by using a specific monoclonal antibody against the PDCoV N protein and an anti-PDCoV rabbit polyclonal antibody. Using DAS-ELISA, the detection limit of recombinant PDCoV N protein and virus titer were approximately 0.5 ng/mL and…
Read MoreMolecularly imprinted polymer as a synthetic antibody for the biorecognition of hazelnut Cor a 14-allergen
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Artificial receptors that mimic their natural biological counterparts have several advantages, such as lower production costs and increased shelf-life stability/versatility, while overcoming the ethical issues related to raising antibodies in animals. In this work, the proposed tailor-made molecularly imprinted polymer (MIP)-allergen receptors aimed at substituting or even transcending the performance of biological antibodies. For this purpose, a MIP…
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Saliva test for COVID-19
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Discussion Our meta-analysis found that the diagnostic sensitivity for saliva NAAT is approximately 83.2% (95% CrI, 74.7%-91.4%), which is comparable to that reported for nasopharyngeal swab NAAT and to the result obtained using our latent class model analysis (84.8%; 95% CrI, 76.8%-92.4%). Given the ease of sample procurement and increased patient comfort, testing centers should strongly…
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Functional rescue in an Angelman syndrome model following treatment with lentivector transduced hematopoietic stem cells
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Angelman Syndrome is a uncommon neurodevelopmental dysfunction characterised by impaired communication expertise, ataxia, motor and steadiness deficits, mental disabilities, and seizures. The genetic reason for Angelman syndrome is the neuronal lack of UBE3A expression within the mind. A novel method, described right here, is a stem cell gene remedy which makes use of lentivector transduced…
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An engineered novel lentivector specifically transducing dendritic cells and eliciting robust HBV-specific CTL response by upregulating autophagy in T cells.
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Dendritic cells (DCs) play a predominant function in initiating cell immune responses. Right here we generated a DC-targeting lentiviral vector (LVDC-UbHBcAg-LIGHT) and evaluated its capability to elicit HBV-specific cytotoxic T lymphocyte (CTL) responses. DC-SIGN-mediated particular transduction utilizing this assemble was confirmed in DC-SIGN-expressing 293T cells and ex vivo-cultured bone marrow cells. LVDC-UbHBcAg-LIGHT-loaded DCs have been…
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Lentivector Knockdown of CCR5 in Hematopoietic Stem and Progenitor Cells Confers Functional and Persistent HIV-1 Resistance in Humanized Mice.
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Gene-engineered CD34(+) hematopoietic stem and progenitor cells (HSPCs) can be utilized to generate an HIV-1-resistant immune system. Nevertheless, a sure threshold of transduced HSPCs is perhaps required for transplantation into mice for creating an HIV-resistant immune system. On this examine, we mixed CCR5 knockdown by a extremely environment friendly microRNA (miRNA) lentivector with pretransplantation number…
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