Category: Synpo
Rat SPON2(Spondin 2) ELISA Kit
- 0
ELISA Kit for the in vitro quantitative measurement of Rat Spondin 2 (SPON2) concentrations in serum, plasma and other biological fluids. This assay has high sensitivity and excellent specificity for detection of Spondin 2 (SPON2) No significant cross-reactivity or interference between Spondin 2 (SPON2) and analogues was observed. The loss rate is less than 5%…
Read MoreAntigen Rapid Test
- 0
COVID-19 (short for “Coronavirus disease 2019”) is a disease first recognized in 2019 that is caused by type of novel coronavirus called SARS-CoV-2. Due to its rapid spread, the World Health Organization (WHO) recognized the disease as a global pandemic on March 11, 2020. Many individuals with confirmed INDICAID COVID-19 have developed fever and/or symptoms…
Read MoreDevelopment of a Novel Double Antibody Sandwich ELISA for Quantitative Detection of Porcine Deltacoronavirus Antigen
- 0
Porcine deltacoronavirus (PDCoV) can cause diarrhea and dehydration in newborn piglets. Here, we developed a double antibody sandwich quantitative enzyme-linked immunosorbent assay (DAS-ELISA) for detection of PDCoV by using a specific monoclonal antibody against the PDCoV N protein and an anti-PDCoV rabbit polyclonal antibody. Using DAS-ELISA, the detection limit of recombinant PDCoV N protein and virus titer were approximately 0.5 ng/mL and…
Read More
An engineered novel lentivector specifically transducing dendritic cells and eliciting robust HBV-specific CTL response by upregulating autophagy in T cells.
- 0
Dendritic cells (DCs) play a predominant function in initiating cell immune responses. Right here we generated a DC-targeting lentiviral vector (LVDC-UbHBcAg-LIGHT) and evaluated its capability to elicit HBV-specific cytotoxic T lymphocyte (CTL) responses. DC-SIGN-mediated particular transduction utilizing this assemble was confirmed in DC-SIGN-expressing 293T cells and ex vivo-cultured bone marrow cells. LVDC-UbHBcAg-LIGHT-loaded DCs have been…
Read More
Direct Lymph Node Vaccination of Lentivector/Prostate-Specific Antigen is Safe and Generates Tissue-Specific Responses in Rhesus Macaques.
- 0
Anti-cancer immunotherapy is rising from a nadir and demonstrating tangible advantages to sufferers. A wide range of approaches are actually employed. We’re invoking antigen (Ag)-specific responses via direct injections of recombinant lentivectors (LVs) that encode sequences for tumor-associated antigens into a number of lymph nodes to optimize immune presentation/stimulation. Right here we first exhibit the…
Read More
Concurrent mutations in RNA-dependent RNA polymerase and spike protein emerged as the epidemiologically most successful SARS-CoV-2 variant
- 0
The D614G mutation within the Spike protein of the SARS-CoV-2 has successfully changed the early pandemic-causing variant. Utilizing pseudotyped lentivectors, we confirmed that the aspartate substitute by glycine in place 614 is markedly extra infectious. Molecular modelling means that the G614 mutation facilitates transition in the direction of an open state of the Spike protein. To…
Read More
Highly efficient ‘hit-and-run’ genome editing with unconcentrated lentivectors carrying Vpr.Prot.Cas9 protein produced from RRE-containing transcripts
- 0
The appliance of gene-editing know-how is presently restricted by the shortage of secure and environment friendly strategies to ship RNA-guided endonucleases to focus on cells. We engineered lentivirus-based nanoparticles to co-package the U6-sgRNA template and the CRISPR-associated protein 9 (Cas9) fused with a virion-targeted protein Vpr (Vpr.Prot.Cas9), for simultaneous supply to cells. Equal spatiotemporal management…
Read More