Tag: protease inhibitor cocktail recipe
Highly efficient ‘hit-and-run’ genome editing with unconcentrated lentivectors carrying Vpr.Prot.Cas9 protein produced from RRE-containing transcripts
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The appliance of gene-editing know-how is presently restricted by the shortage of secure and environment friendly strategies to ship RNA-guided endonucleases to focus on cells. We engineered lentivirus-based nanoparticles to co-package the U6-sgRNA template and the CRISPR-associated protein 9 (Cas9) fused with a virion-targeted protein Vpr (Vpr.Prot.Cas9), for simultaneous supply to cells. Equal spatiotemporal management…
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