Rapid C.Brucella Ab Test Kit

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Brucellosis, also known as undulant fever, Mediterranean fever is a zoonosis and the infection is almost invariably transmitted by direct or indirect contact with infected animals or their products. Expansion of animal industries and urbanization, and the lack of hygienic measures in animal husbandry and in food handling partly account for brucellosis remaining a public health hazard. Expansion of international travel which stimulates the taste for exotic dairy goods such as fresh cheeses which may be contaminated, and the importation of such foods into Brucella-free regions, also contribute to the ever-increasing concern over human brucellosis. The GENPRICE GENTAUR Rapid C.Brucella Ab Test kit is based on sandwich immunochromatographic assay, to detect bovine brucellosis antibody in serum / plasma / blood, which is rapid, accurate and easy-to-operate.

Introduction

Brucella canis is sexually transmitted by the mating of infected males and females. Brucella canis in the female dog will live in the vaginal and uterine tissue and secretions for years, usually for life. In males, the Brucella bacteria live in the testicles and seminal fluids.
An infected male is just as contagious as the female since he can spread the Brucella bacteria via urine or semen. Transmission between dogs occurs via mucous membranes, so the bacteria may enter the body through the nose, mouth, conjunctiva of the eye and vagina.
The majority of bacteria in infected dogs are secreted in semen and vaginal secretions. Bacteria may also be present in milk, urine and saliva, thus any body fluids can infect another dog.
When an infected bitch aborts, spread throughout a kennel can be very rapid. Infected bitches may deliver both living and dead puppies. These surviving puppies are infected and will shed bacteria in their secretions.

WHAT IS THE IMMUNOCOMB ASSAY ?

The ImmunoComb test is a modified ELISA, which can be described as an enzyme labeled “dot assay”, that detects antibody levels in serum, plasma or whole blood. The kit contains all the necessary reagents for developing the test. Results for the Brucella canis tests are obtained within 23 minutes.

HOW DOES THE IMMUNOCOMB WORK?

■ The ImmunoComb Kit contains 2 main components: a comb shaped plastic card, hereafter
referred to as the Comb and a multi compartment developing plate.
■ The Comb has 12 teeth – sufficient for 12 tests. Each tooth will be developed in a corresponding column of wells in the developing plate. Individual or multiple tests are processed by breaking off the
desired number of teeth from the Comb.
■ A test spot of Brucella antigen is attached to the lowest spot of each tooth on the Comb. The upper
most spot is a Positive Reference.
■ The first step of the test is to deposit a serum, plasma or whole blood specimen into a well in row
A of the multi-compartment developing plate.
■ Next, the Comb is inserted into the well(s) with the sample(s) and transferred to the remaining wells
(B-F) at timed intervals, according to the step by step instructions (see section VII). Specific IgG antibodies from the specimen, if present, bind to the antigen
at the test spots and will be labeled in row C, which contains an enzyme labeled anti-dog IgG antibody.
■ At the end of the developing process, a purplegrey color results are developed in all Positive Reference spots and in any positive sample tested spot.
■ The intensity of the color result corresponds directly to the antibody level in the test specimen. Results are scored using the Positive Reference spot and CombScale.

Kit Components

Test Cassette, 50 pcs
Sample buffer, 2 pcs
Disposable pipette, 50 pcs
Kit instruction, 1 pc

Special features

– Earlier detection of C. Brucella Ab than blood culture and 2-ME RSAT
– World’s first & sole C.brucella Ab Rapid Test Kit
– One step testing procedure: Easy to use, Saving time and labor
– No additional equipment is required.

Specifications
– Principle: lmmunochromatographic assay
– (LPS capture)-(blood)-(Monoclonal anti-canine lgG detector) = LPS (Lipopolysaccharide) antigen originated from Brucella canis
– Purpose: Detection of Brucella canins antibody
– Specimen: Canine whole blood, plasma, or serum.

– Reading time: 20 minutes
– Sensitivity: 93% vs. blood culture
– Specificity: 100% vs. blood culture
– Shelf life: 24 months
– Storage temperature: 2-30ºC.