
Direct Lymph Node Vaccination of Lentivector/Prostate-Specific Antigen is Safe and Generates Tissue-Specific Responses in Rhesus Macaques.
Crystal
- 0
Anti-cancer immunotherapy is rising from a nadir and demonstrating tangible advantages to sufferers. A wide range of approaches are actually employed. We’re invoking antigen (Ag)-specific responses via direct injections of recombinant lentivectors (LVs) that encode sequences for tumor-associated antigens into a number of lymph nodes to optimize immune presentation/stimulation.
Right here we first exhibit the effectiveness and antigen-specificity of this strategy in mice challenged with prostate-specific antigen (PSA)-expressing tumor cells. Subsequent we examined the protection and efficacy of this strategy in two cohorts of rhesus macaques as a prelude to a medical trial utility.
Our vector encodes the cDNA for rhesus macaque PSA and a rhesus macaque cell floor marker to facilitate vector titering and monitoring. We utilized two unbiased injection schemas demarcated by the timing of LV administration. In each cohorts we noticed marked tissue-specific responses as measured by medical evaluations and magnetic resonance imaging of the prostate gland.
Tissue-specific responses had been sustained for as much as six months-the end-point of the research. Management animals immunized towards an irrelevant Ag had been unaffected. We didn’t observe vector unfold in take a look at or management animals or perturbations of systemic immune parameters. This strategy thus gives an “off-the-shelf” anti-cancer vaccine that could possibly be made at giant scale and injected into patients-even on an out-patient foundation.
Intratumoral Lentivector-Mediated TGF-β1 Gene Downregulation As a Potent Technique for Enhancing the Antitumor Impact of Remedy Composed of Cyclophosphamide and Dendritic Cells.
Vaccination with dendritic cells (DCs) stimulated with tumor antigens can induce particular mobile immune response that acknowledges a excessive spectrum of tumor antigens. Nevertheless, the flexibility of most cancers cells to provide immunosuppressive components drastically decreases the antitumor exercise of DCs.
The principle objective of the research was to enhance the effectiveness of DC-based immunotherapy or chemoimmunotherapy composed of cyclophosphamide (CY) and DCs by utility of lentivectors (LVs)-encoding brief hairpin RNA particular for TGF-β1 (shTGFβ1 LVs). We noticed that s.c. inoculation of each MC38 cells with silenced expression of TGF-β1 (MC38/shTGF-β1) and direct intratumoral utility of shTGFβ1 LVs contributed to discount of suppressor exercise of myeloid cells and Tregs in tumor.
Opposite to expectations, in mice bearing wild tumor, the applying of shTGFβ1 LVs previous to vaccination with bone marrow-derived DC stimulated with tumor antigens (BMDC/TAg) didn’t affect myeloid-derived suppressor cell (MDSC) infiltration into tumor. Because of this, we noticed solely minor MC38 tumor development inhibition (TGI) accompanied by systemic antitumor response activation similar to that obtained for destructive management (shN).
Nevertheless, when the proposed scheme was complemented by pretreatment with a low dose of CY, we seen excessive MC38 TGI along with decreased variety of MDSCs in tumor and induction of Th1-type response. Furthermore, in each schemes of remedy, LVs (shTGFβ1 in addition to shN) induced excessive inflow of CTLs into tumor related in all probability with the viral antigen introduction into tumor microenvironment. Concluding, the applying of shTGFβ1 LVs alone or together with DC-based vaccines just isn’t adequate for long-lasting elimination of suppression in tumor.
Nevertheless, simultaneous discount of TGF-β1 in tumor microenvironment and its transforming by pretreatment with a low dose of CY facilitates the settlement of peritumorally inoculated DCs and helps them in restoration and activation of a potent antitumor response.

Dendritic Cells Cross-Current Immunogenic Lentivector-Encoded Antigen from Transduced Cells to Prime Purposeful T Cell Immunity.
Recombinant lentiviral vectors (LVs) are extremely efficient vaccination autos that elicit protecting T cell immunity in illness fashions. Dendritic cells (DCs) purchase antigen at websites of vaccination and migrate to draining lymph nodes, the place they prime vaccine-specific T cells. The efficiency with which LVs activate CD8+ T cell immunity has been attributed to the transduction of DCs on the immunization website and sturdy presentation of LV-encoded antigens.
Nevertheless, it’s not recognized how LV-encoded antigens proceed to be offered to T cells as soon as straight transduced DCs have turned over. Right here, we report that LV-encoded antigen is effectively cross-presented by DCs in vitro. We have now additional exploited the temporal depletion of DCs within the murine CD11c.DTR (diphtheria toxin receptor) mannequin to exhibit that repopulating DCs that had been absent on the time of immunization cross-present LV-encoded antigen to T cells in vivo.
Oblique presentation of antigen from transduced cells by DCs is adequate to prime practical effector T cells that management tumor development. These information recommend that DCs cross-present immunogenic antigen from LV-transduced cells, thereby facilitating extended activation of T cells within the absence of circulating LV particles. These are findings which will affect on the longer term design of LV vaccination methods.
Optimization of a Genome-Broad Disordered Lentivector-Primarily based Brief Hairpin RNA Library.
To acquire an entire genome library that suppresses the full variety of human mRNAs, lentiviral vector constructs and a brief hairpin RNA (shRNA) expression cassette had been optimized. The optimization of the vector elevated the virus titer in preparations by 15-20 instances. A easy shRNA construction with a 21-bp stem proved to be the simplest.
Lentivector-based shRNA expression constructs had been obtained through the use of puro(R), copGFP, or H-2K(okay) as a selectable marker. The effectivity of the optimized library was demonstrated when screening for shRNAs reactivating the tumor suppressor p53 in HeLa cells. Cells carried a reporter assemble guaranteeing p53-responsive synthesis of a fluorescent protein, which allowed choice of cells with reactivated p53 by stream cytometry.
Injectable alginate hydrogel for enhanced spatiotemporal management of lentivector supply in murine skeletal muscle.
Hydrogels are an particularly interesting class of biomaterials for gene supply autos as they are often launched into the physique with minimally invasive procedures and are sometimes utilized in tissue engineering and regenerative medication methods. On this research, we present for the primary time using an injectable alginate hydrogel for managed supply of lentivectors within the skeletal muscle of murine hindlimb. We suggest to change the discharge charges of lentivectors via manipulation of the molecular weight distribution of alginate hydrogels.
The discharge of lentivector was examined utilizing two completely different ratios of high and low molecular weight (MW) alginate polymers (75/25 and 25/75 low/excessive MW). The interdependency of lentivector launch fee and alginate degradation fee was assessed in vitro. Lentivector-loaded hydrogels maintained transduction potential for as much as one week in vitro as demonstrated by the continuous transduction of HEK-293T cells.
pENTR223-CENPK |
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PVT12726 | Lifescience Market | 2 ug | EUR 469.2 |
CENPK Polyclonal Antibody |
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ABP58115-003ml | Abbkine | 0.03ml | EUR 189.6 |
Description: A polyclonal antibody for detection of CENPK from Human, Mouse. This CENPK antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human CENPK protein at amino acid sequence of 50-130 |
CENPK Polyclonal Antibody |
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ABP58115-01ml | Abbkine | 0.1ml | EUR 346.8 |
Description: A polyclonal antibody for detection of CENPK from Human, Mouse. This CENPK antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human CENPK protein at amino acid sequence of 50-130 |
CENPK Polyclonal Antibody |
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ABP58115-02ml | Abbkine | 0.2ml | EUR 496.8 |
Description: A polyclonal antibody for detection of CENPK from Human, Mouse. This CENPK antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human CENPK protein at amino acid sequence of 50-130 |
CENPK Rabbit pAb |
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A7627-100ul | Abclonal | 100 ul | EUR 369.6 |
CENPK Rabbit pAb |
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A7627-200ul | Abclonal | 200 ul | EUR 550.8 |
CENPK Rabbit pAb |
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A7627-20ul | Abclonal | 20 ul | EUR 219.6 |
CENPK Rabbit pAb |
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A7627-50ul | Abclonal | 50 ul | EUR 267.6 |
CENPK Conjugated Antibody |
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C45679 | SAB | 100ul | EUR 476.4 |
CENPK cloning plasmid |
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CSB-CL005214HU-10ug | Cusabio | 10ug | EUR 279.6 |
Description: A cloning plasmid for the CENPK gene. |
CENPK Blocking Peptide |
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DF9059-BP | Affbiotech | 1mg | EUR 234 |
CENPK Polyclonal Antibody |
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ES9253-100ul | ELK Biotech | 100ul | EUR 334.8 |
Description: A Rabbit Polyclonal antibody against CENPK from Human/Mouse. This antibody is tested and validated for WB, ELISA, WB, ELISA |
CENPK Polyclonal Antibody |
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ES9253-50ul | ELK Biotech | 50ul | EUR 248.4 |
Description: A Rabbit Polyclonal antibody against CENPK from Human/Mouse. This antibody is tested and validated for WB, ELISA, WB, ELISA |
Anti-CENPK antibody |
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STJ29764 | St John's Laboratory | 100 µl | EUR 332.4 |
Anti-CENPK antibody |
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STJ190411 | St John's Laboratory | 200 µl | EUR 236.4 |
Description: Unconjugated Rabbit polyclonal to CENPK |
Human CENPK shRNA Plasmid |
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20-abx961921 | Abbexa |
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Mouse CENPK shRNA Plasmid |
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20-abx975206 | Abbexa |
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CENPK Recombinant Protein (Human) |
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RP006757 | ABM | 100 ug | Ask for price |
CENPK Recombinant Protein (Rat) |
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RP194543 | ABM | 100 ug | Ask for price |
CENPK Recombinant Protein (Mouse) |
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RP123488 | ABM | 100 ug | Ask for price |
CENPK Recombinant Protein (Mouse) |
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RP123491 | ABM | 100 ug | Ask for price |
Centromere Protein K (CENPK) Antibody |
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20-abx149226 | Abbexa |
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Centromere Protein K (CENPK) Antibody |
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20-abx006672 | Abbexa |
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Centromere Protein K (CENPK) Antibody |
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20-abx321953 | Abbexa |
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Centromere Protein K (CENPK) Antibody |
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20-abx321954 | Abbexa |
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Human Centromere protein K (CENPK) |
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1-CSB-EP005214HU | Cusabio |
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Description: Recombinant Human Centromere protein K(CENPK) expressed in E.coli |
CENPK ELISA Kit (Human) (OKCA00632) |
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OKCA00632 | Aviva Systems Biology | 96 Wells | EUR 999.6 |
Description: Description of target: Component of the CENPA-CAD (nucleosome distal) complex, a complex recruited to centromeres which is involved in assembly of kinetochore proteins, mitotic progression and chromosome segregation. May be involved in incorporation of newly synthesized CENPA into centromeres via its interaction with the CENPA-NAC complex. Acts in coordination with CASC5/KNL1 to recruit the NDC80 complex to the outer kinetochore.;Species reactivity: Human;Application: ;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 5.8 pg/mL |
Cenpk ORF Vector (Rat) (pORF) |
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ORF064849 | ABM | 1.0 ug DNA | EUR 607.2 |
CENPK ORF Vector (Human) (pORF) |
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ORF002253 | ABM | 1.0 ug DNA | EUR 114 |
Cenpk ORF Vector (Mouse) (pORF) |
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ORF041164 | ABM | 1.0 ug DNA | EUR 607.2 |
Cenpk ORF Vector (Mouse) (pORF) |
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ORF041165 | ABM | 1.0 ug DNA | EUR 607.2 |
Rabbit Anti-Human CENPK polyclonal antibody |
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CABT-BL124 | Creative Diagnostics | 100 ul | EUR 702 |
CENPK sgRNA CRISPR Lentivector set (Human) |
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K0432501 | ABM | 3 x 1.0 ug | EUR 406.8 |
Cenpk sgRNA CRISPR Lentivector set (Rat) |
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K6564001 | ABM | 3 x 1.0 ug | EUR 406.8 |
Cenpk sgRNA CRISPR Lentivector set (Mouse) |
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K3101501 | ABM | 3 x 1.0 ug | EUR 406.8 |
Human Centromere protein K(CENPK) ELISA kit |
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CSB-EL005214HU-24T | Cusabio | 1 plate of 24 wells | EUR 198 |
Description: Quantitativesandwich ELISA kit for measuring Human Centromere protein K (CENPK) in samples from serum, plasma, tissue homogenates, cell lysates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price. |
Human Centromere protein K(CENPK) ELISA kit |
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1-CSB-EL005214HU | Cusabio |
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Description: Quantitativesandwich ELISA kit for measuring Human Centromere protein K(CENPK) in samples from serum, plasma, tissue homogenates, cell lysates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits. |
Goat Centromere protein K(CENPK) ELISA kit |
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E06C0931-192T | BlueGene | 192 tests | EUR 1524 |
Description: A competitive ELISA for quantitative measurement of Goat Centromere protein K(CENPK) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Goat Centromere protein K(CENPK) ELISA kit |
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E06C0931-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
Description: A competitive ELISA for quantitative measurement of Goat Centromere protein K(CENPK) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Goat Centromere protein K(CENPK) ELISA kit |
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E06C0931-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
Description: A competitive ELISA for quantitative measurement of Goat Centromere protein K(CENPK) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rabbit Centromere protein K(CENPK) ELISA kit |
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E04C0931-192T | BlueGene | 192 tests | EUR 1524 |
Description: A competitive ELISA for quantitative measurement of Rabbit Centromere protein K(CENPK) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rabbit Centromere protein K(CENPK) ELISA kit |
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E04C0931-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
Description: A competitive ELISA for quantitative measurement of Rabbit Centromere protein K(CENPK) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rabbit Centromere protein K(CENPK) ELISA kit |
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E04C0931-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
Description: A competitive ELISA for quantitative measurement of Rabbit Centromere protein K(CENPK) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Dog Centromere protein K(CENPK) ELISA kit |
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E08C0931-192T | BlueGene | 192 tests | EUR 1524 |
Description: A competitive ELISA for quantitative measurement of Canine Centromere protein K(CENPK) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Dog Centromere protein K(CENPK) ELISA kit |
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E08C0931-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
Description: A competitive ELISA for quantitative measurement of Canine Centromere protein K(CENPK) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Dog Centromere protein K(CENPK) ELISA kit |
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E08C0931-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
Description: A competitive ELISA for quantitative measurement of Canine Centromere protein K(CENPK) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Monkey Centromere protein K(CENPK) ELISA kit |
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E09C0931-192T | BlueGene | 192 tests | EUR 1524 |
Description: A competitive ELISA for quantitative measurement of Monkey Centromere protein K(CENPK) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Monkey Centromere protein K(CENPK) ELISA kit |
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E09C0931-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
Description: A competitive ELISA for quantitative measurement of Monkey Centromere protein K(CENPK) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Monkey Centromere protein K(CENPK) ELISA kit |
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E09C0931-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
Description: A competitive ELISA for quantitative measurement of Monkey Centromere protein K(CENPK) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Human Centromere protein K(CENPK) ELISA kit |
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E01C0931-192T | BlueGene | 192 tests | EUR 1524 |
Description: A competitive ELISA for quantitative measurement of Human Centromere protein K(CENPK) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Human Centromere protein K(CENPK) ELISA kit |
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E01C0931-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
Description: A competitive ELISA for quantitative measurement of Human Centromere protein K(CENPK) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Human Centromere protein K(CENPK) ELISA kit |
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E01C0931-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
Description: A competitive ELISA for quantitative measurement of Human Centromere protein K(CENPK) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rat Centromere protein K(CENPK) ELISA kit |
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E02C0931-192T | BlueGene | 192 tests | EUR 1524 |
Description: A competitive ELISA for quantitative measurement of Rat Centromere protein K(CENPK) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rat Centromere protein K(CENPK) ELISA kit |
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E02C0931-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
Description: A competitive ELISA for quantitative measurement of Rat Centromere protein K(CENPK) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rat Centromere protein K(CENPK) ELISA kit |
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E02C0931-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
Description: A competitive ELISA for quantitative measurement of Rat Centromere protein K(CENPK) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Mouse Centromere protein K(CENPK) ELISA kit |
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E03C0931-192T | BlueGene | 192 tests | EUR 1524 |
Description: A competitive ELISA for quantitative measurement of Mouse Centromere protein K(CENPK) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Mouse Centromere protein K(CENPK) ELISA kit |
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E03C0931-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
Description: A competitive ELISA for quantitative measurement of Mouse Centromere protein K(CENPK) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Mouse Centromere protein K(CENPK) ELISA kit |
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E03C0931-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
Description: A competitive ELISA for quantitative measurement of Mouse Centromere protein K(CENPK) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Human Centromere protein K, CENPK ELISA KIT |
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ELI-34111h | Lifescience Market | 96 Tests | EUR 988.8 |
Chicken Centromere protein K, CENPK ELISA KIT |
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ELI-34204c | Lifescience Market | 96 Tests | EUR 1113.6 |
Pig Centromere protein K(CENPK) ELISA kit |
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E07C0931-192T | BlueGene | 192 tests | EUR 1524 |
Description: A competitive ELISA for quantitative measurement of Porcine Centromere protein K(CENPK) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Pig Centromere protein K(CENPK) ELISA kit |
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E07C0931-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
Description: A competitive ELISA for quantitative measurement of Porcine Centromere protein K(CENPK) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Pig Centromere protein K(CENPK) ELISA kit |
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E07C0931-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
Description: A competitive ELISA for quantitative measurement of Porcine Centromere protein K(CENPK) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Mouse Centromere protein K, Cenpk ELISA KIT |
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ELI-11052m | Lifescience Market | 96 Tests | EUR 1038 |
CENPK sgRNA CRISPR Lentivector (Human) (Target 1) |
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K0432502 | ABM | 1.0 ug DNA | EUR 184.8 |
CENPK sgRNA CRISPR Lentivector (Human) (Target 2) |
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K0432503 | ABM | 1.0 ug DNA | EUR 184.8 |
CENPK sgRNA CRISPR Lentivector (Human) (Target 3) |
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K0432504 | ABM | 1.0 ug DNA | EUR 184.8 |
Cenpk sgRNA CRISPR Lentivector (Rat) (Target 1) |
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K6564002 | ABM | 1.0 ug DNA | EUR 184.8 |
Cenpk sgRNA CRISPR Lentivector (Rat) (Target 2) |
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K6564003 | ABM | 1.0 ug DNA | EUR 184.8 |
Cenpk sgRNA CRISPR Lentivector (Rat) (Target 3) |
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K6564004 | ABM | 1.0 ug DNA | EUR 184.8 |
Cenpk sgRNA CRISPR Lentivector (Mouse) (Target 1) |
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K3101502 | ABM | 1.0 ug DNA | EUR 184.8 |
Cenpk sgRNA CRISPR Lentivector (Mouse) (Target 2) |
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K3101503 | ABM | 1.0 ug DNA | EUR 184.8 |
Cenpk sgRNA CRISPR Lentivector (Mouse) (Target 3) |
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K3101504 | ABM | 1.0 ug DNA | EUR 184.8 |
CENPK Protein Vector (Human) (pPB-C-His) |
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PV009009 | ABM | 500 ng | EUR 394.8 |
CENPK Protein Vector (Human) (pPB-N-His) |
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PV009010 | ABM | 500 ng | EUR 394.8 |
CENPK Protein Vector (Human) (pPM-C-HA) |
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PV009011 | ABM | 500 ng | EUR 394.8 |
CENPK Protein Vector (Human) (pPM-C-His) |
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PV009012 | ABM | 500 ng | EUR 394.8 |
CENPK Protein Vector (Mouse) (pPB-C-His) |
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PV164654 | ABM | 500 ng | EUR 723.6 |
CENPK Protein Vector (Mouse) (pPB-N-His) |
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PV164655 | ABM | 500 ng | EUR 723.6 |
CENPK Protein Vector (Mouse) (pPM-C-HA) |
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PV164656 | ABM | 500 ng | EUR 723.6 |
CENPK Protein Vector (Mouse) (pPM-C-His) |
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PV164657 | ABM | 500 ng | EUR 723.6 |
CENPK Protein Vector (Mouse) (pPB-C-His) |
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PV164658 | ABM | 500 ng | EUR 723.6 |
CENPK Protein Vector (Mouse) (pPB-N-His) |
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PV164659 | ABM | 500 ng | EUR 723.6 |
CENPK Protein Vector (Mouse) (pPM-C-HA) |
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PV164660 | ABM | 500 ng | EUR 723.6 |
CENPK Protein Vector (Mouse) (pPM-C-His) |
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PV164661 | ABM | 500 ng | EUR 723.6 |
CENPK 3'UTR Luciferase Stable Cell Line |
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TU004223 | ABM | 1.0 ml | EUR 1672.8 |
Cenpk 3'UTR Luciferase Stable Cell Line |
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TU103719 | ABM | 1.0 ml | Ask for price |
CENPK 3'UTR GFP Stable Cell Line |
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TU054223 | ABM | 1.0 ml | EUR 1672.8 |
Cenpk 3'UTR Luciferase Stable Cell Line |
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TU202175 | ABM | 1.0 ml | Ask for price |
Cenpk 3'UTR GFP Stable Cell Line |
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TU252175 | ABM | 1.0 ml | Ask for price |
Cenpk 3'UTR GFP Stable Cell Line |
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TU153719 | ABM | 1.0 ml | Ask for price |
CENPK Protein Vector (Rat) (pPB-C-His) |
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PV259394 | ABM | 500 ng | EUR 723.6 |
CENPK Protein Vector (Rat) (pPB-N-His) |
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PV259395 | ABM | 500 ng | EUR 723.6 |
CENPK Protein Vector (Rat) (pPM-C-HA) |
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PV259396 | ABM | 500 ng | EUR 723.6 |
CENPK Protein Vector (Rat) (pPM-C-His) |
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PV259397 | ABM | 500 ng | EUR 723.6 |
Guinea pig Centromere protein K(CENPK) ELISA kit |
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E05C0931-192T | BlueGene | 192 tests | EUR 1524 |
Description: A competitive ELISA for quantitative measurement of Guinea pig Centromere protein K(CENPK) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Guinea pig Centromere protein K(CENPK) ELISA kit |
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E05C0931-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
Description: A competitive ELISA for quantitative measurement of Guinea pig Centromere protein K(CENPK) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Guinea pig Centromere protein K(CENPK) ELISA kit |
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E05C0931-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
Description: A competitive ELISA for quantitative measurement of Guinea pig Centromere protein K(CENPK) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
CENPK sgRNA CRISPR/Cas9 All-in-One Lentivector set (Human) |
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K0432505 | ABM | 3 x 1.0 ug | EUR 451.2 |
Cenpk sgRNA CRISPR/Cas9 All-in-One Lentivector set (Rat) |
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K6564005 | ABM | 3 x 1.0 ug | EUR 451.2 |
Cenpk sgRNA CRISPR/Cas9 All-in-One Lentivector set (Mouse) |
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K3101505 | ABM | 3 x 1.0 ug | EUR 451.2 |
CENPK sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 1) |
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K0432506 | ABM | 1.0 ug DNA | EUR 200.4 |
CENPK sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 2) |
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K0432507 | ABM | 1.0 ug DNA | EUR 200.4 |
CENPK sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 3) |
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K0432508 | ABM | 1.0 ug DNA | EUR 200.4 |
Cenpk sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 1) |
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K6564006 | ABM | 1.0 ug DNA | EUR 200.4 |
Cenpk sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 2) |
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K6564007 | ABM | 1.0 ug DNA | EUR 200.4 |
Cenpk sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 3) |
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K6564008 | ABM | 1.0 ug DNA | EUR 200.4 |
Cenpk sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 1) |
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K3101506 | ABM | 1.0 ug DNA | EUR 200.4 |
Cenpk sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 2) |
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K3101507 | ABM | 1.0 ug DNA | EUR 200.4 |
Cenpk sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 3) |
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K3101508 | ABM | 1.0 ug DNA | EUR 200.4 |
GFP (Bsd) lentiviral particles |
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LVP001 | GenTarget | 1x10e7 IFU/ml x 200ul | EUR 418.8 |
Description: Pre-made lentiviral particles expressing eGFP with Blasticidin antibiotic marker, provided in DMEM medium with 10% FBS. |
CFP (Bsd) lentiviral particles |
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LVP011 | GenTarget | 1x10e7 IFU/ml x 200ul | EUR 418.8 |
Description: Pre-made lentiviral particles expressing cyan fluorescent protein (CFP), provided in DMEM containing 10% FBS and 60ug/ml polybrene. |
YFP (Bsd) lentiviral particles |
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LVP012 | GenTarget | 1x10e7 IFU/ml x 200ul | EUR 418.8 |
Description: Pre-made lentiviral particles expressing yellow fluorescent protein (YFP), rovided in DMEM medium with 10% FBS, 60ug/ml polybrene. |
TK (His) Lentiviral particles |
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LVP018 | GenTarget | 1x107 IFU/ml x 200ul | EUR 418.8 |
Description: pre-made lentiviral particles expressing Thymidine kinase gene with N-term His tag |
TAT (His) Lentiviral particles |
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LVP019 | GenTarget | 5x107 IFU/ml x 200ul | EUR 418.8 |
Description: pre-made lentiviral particles expressing HIV TAT gene with N-term His tag |
RFP (Bsd) Lentiviral particles |
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LVP023 | GenTarget | 1x10e7 IFU/ml x 200ul | EUR 418.8 |
Description: Pre-made lentiviral particles expressing RFP with Blasticidin antibiotic marker, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene. |
RFP (Neo) lentiviral particles |
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LVP299 | GenTarget | 1x10e7 IFU/ml x 200ul | EUR 418.8 |
Description: Pre-made lentiviral particles expressing RFP with Neomycin antibiotic marker, in DMEM medium with 10% FBS and 60ug/ml of polybrene. |
GFP (Neo) lentiviral particles |
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LVP300 | GenTarget | 1x10e7 IFU/ml x 200ul | EUR 418.8 |
Description: Pre-made lentiviral particles expressing GFP with Neomycin antibiotic marker, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene. |
lacZ (Puro) lentiviral particles |
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LVP301 | GenTarget | 1x107 IFU/ml x 200ul | EUR 541.2 |
Description: Pre-made lentiviral particles expressing full length lacZ enzyme (β-Galactosidase) with puromycin marker, provided in DMDM medium with 10% FBS. |
CFP (Neo) lentiviral particles |
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LVP306 | GenTarget | 1x10e7 IFU/ml x 200ul | EUR 418.8 |
Description: Pre-made lentiviral particles expressing CFP with Neomycin antibiotic marker, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene. |
YFP (Neo) lentiviral particles |
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LVP307 | GenTarget | 1x10e7 IFU/ml x 200ul | EUR 418.8 |
Description: Pre-made lentiviral particles expressing YFP with Neomycin antibiotic marker, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene. |
RFP (Puro) lentiviral particles |
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LVP309 | GenTarget | 1x10e7 IFU/ml x 200ul | EUR 418.8 |
Description: Pre-made RFP lentiviral particles. RFP was expressed under suCMV promoter. A puromycin marker was expressed under RSV promoter. Provided in DMEM medium with 10% FBS and 60ug/ml of polybrene. |
lacZ (Neo) lentiviral particles |
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LVP333 | GenTarget | 1x107 IFU/ml x 200ul | EUR 541.2 |
Description: Pre-made lentiviral particles expressing full length lacZ enzyme (β-Galactosidase) with Neomycin marker, provided in DMDM medium with 10% FBS. |
lacZ (luciferase) lentiviral particles |
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LVP334 | GenTarget | 1x107 IFU/ml x 200ul | EUR 541.2 |
Description: Pre-made lentiviral particles expressing full length lacZ enzyme (β-Galactosidase) with luciferase marker, provided in DMDM medium with 10% FBS. |
GFP (puro) lentiviral particles |
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LVP340 | GenTarget | 1x10e7 IFU/ml x 200ul | EUR 418.8 |
Description: Pre-made lentiviral particles expressing GFP with Puromycin antibiotic marker, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene. |
CFP (puro) lentiviral particles |
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LVP342 | GenTarget | 1x10e7 IFU/ml x 200ul | EUR 418.8 |
Description: Pre-made lentiviral particles expressing CFP with Puromycin antibiotic marker, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene. |
lacZ (Bsd) lentiviral particles |
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LVP346 | GenTarget | 1x107 IFU/ml x 200ul | EUR 541.2 |
Description: Pre-made lentiviral particles expressing full length lacZ enzyme (β-Galactosidase) with blasticidin marker, provided in DMDM medium with 10% FBS. |
rtTA (Bsd) , Lentiviral particles |
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LVP400-Bsd | GenTarget | 1x107 IFU/ml x 200ul | EUR 484.8 |
Description: Pre-made lentiviral particles expressing reverse tetracycline Transcriptional Activator (rtTA) under enhanced EF1a promoter with Blasticidin antibioic marker, provided in DMEM medium with 10% FBS. |
rtTA (Neo) , Lentiviral particles |
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LVP400-Neo | GenTarget | 1x107 IFU/ml x 200ul | EUR 484.8 |
Description: Pre-made lentiviral particles expressing reverse tetracycline Transcriptional Activator (rtTA) under enhanced EF1a promoter with Neomycin antibioic marker, provided in DMEM medium with 10% FBS. |
rtTA (Puro) , Lentiviral particles |
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LVP400-Puro | GenTarget | 1x107 IFU/ml x 200ul | EUR 484.8 |
Description: Pre-made lentiviral particles expressing reverse tetracycline Transcriptional Activator (rtTA) under enhanced EF1a promoter with Puromycin antibioic marker, provided in DMEM medium with 10% FBS. |
×
Injection of lentivector-loaded hydrogel in vivo led to a sustained stage of transgene expression for greater than two months whereas minimizing the copies of lentivirus genome inserted into the genome of murine skeletal muscle cells. This technique of spatiotemporal management of lentivector supply from alginate hydrogels might present a flexible instrument to mix gene remedy and biomaterials for functions in regenerative medication.